بررسی سطح پلاسمائی هوموسیستئین در موشهای کلستاتیک و تأثیر آن بر ترشح نیتریک اکساید در کبد

Homocystein &nbsp;(Hcy),one of the thio-amino acid is known as a risk &nbsp;&nbsp;factor&nbsp;in some cardiovascular diseases with releasing O2 radical . It has also been&nbsp;reported that; there is oxidative stress effects of Hcy in cholestasis. The aim of&nbsp;this study is to determine plasma Hcy alteration and nitric oxide (NO) in liver and its &nbsp;&nbsp; effects on pathologic disfunction. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; In this study , 150 Spraque &ndash; Dawley male rats with 200 &plusmn; 20g body weight&nbsp;were used in the experiments and they were randomly divided in three control,&nbsp;SHAM and bile duct ligation (BDL) groups (n= 10-12) . In 7th,14th,21st and 28th days&nbsp;cholestasis was observed in BDL group,the animal were anesthetized with ether &nbsp;&nbsp; and then blood samples were taken from heart directly and analysed for cystein ,&nbsp;methionine by HPLC and&nbsp;HPLC-UV. Two hours before blood sampling , 40 and 100&nbsp;mg/kg methionine were injected (I.P) . All data are expressed as mean &plusmn; SEM. Statistical evaluation of data&nbsp;performed by SPSS soft ware using analysis of variance (ANOVA) followed by post&nbsp;hoc test. P-values less than 0.05 were considered statistically significant . The results suggest that billirubin and hepatic enzymes were significantly&nbsp;elevated in BDL rats compared with SHAM and controls (P<0.05). Homocystein&nbsp;concentration was significantly rised in 14th day in BDL group (P<0.05). The plasma&nbsp;cystein and methionine level were significantly elevated in BDL rats compared with&nbsp;SHAM and control groups ( p = 0.01) . Plasma nitrate / nitrite ratio were significantly&nbsp;increased in BDL rats compared with SHAM and control rats&nbsp;(P<0.05). With these&nbsp;data we suppose that some of the systemic oxidative stresses in BDL rat model of&nbsp;&nbsp; cholestasis contributes possibly through NO-dependent mechanisms disorders.